{"podcast":{"title":"Dr. John Campbell","slug":"dr-john-campbell-7851784","podcast_index_feed_id":7851784,"rss_url":"https://feeds.megaphone.fm/VG9554039150","website_url":null,"image_url":"https://megaphone.imgix.net/podcasts/7a133470-32e4-11f1-ae26-ef5a830a5f06/image/aacd596ff9b51467520b0238cfcd1c87.png?ixlib=rails-4.3.1&max-w=3000&max-h=3000&fit=crop&auto=format,compress","author":"Campbellteaching","episode_count":480,"summary":"Hello Everyone, My name is John Campbell and I am a retired Nurse Teacher and former clinical nurse based in England. I also do some teaching in Asia and Africa when time permits. These videos are to help students to learn the background to all forms of health care. My PhD focused on the development of open learning resources for nurses nationally and internationally. LinkedIn profile, https://www.linkedin.com/in/dr-john-campbell-5256223b/ Twitter, https://twitter.com/Johnincarlisle Disclaimer; These media including videos, book, e book, articles, podcasts are not peer-reviewed. They should never replace individual clinical judgement from your own health care provider. No media-based material on this channel is suitable for using as professional medical advice. All comments are also for educational purposed only and must never replace advice from your own health care provider","last_synced_at":"2026-07-23T10:19:57.883522+00:00","page_url":"https://stenobird.com/podcast/dr-john-campbell-7851784"},"episode":{"title":"Huge DNA contamination","slug":"huge-dna-contamination","published_at":"2026-07-13T01:30:00+00:00","page_url":"https://stenobird.com/podcast/dr-john-campbell-7851784/huge-dna-contamination","show_page_url":"https://stenobird.com/podcast/dr-john-campbell-7851784","url":"https://traffic.megaphone.fm/VG6622757007.mp3","audio_url":"https://traffic.megaphone.fm/VG6622757007.mp3","summary":"Quantification of residual plasmid DNA and SV40 promoter-enhancer sequences in Pfizer/BioNTech and Moderna modRNA COVID-19 vaccines from Ontario, Canada https://pubmed.ncbi.nlm.nih.gov/40913499/ 32 vials representing 16 unique vaccine lots. These data demonstrate the presence of billions to hundreds of billions of DNA molecules per dose in the modRNA COVID-19 products tested. All products tested exceeded the guidelines for residual DNA set by the FDA and WHO of 10ng/dose by 36–627-fold. Total DNA in all vials tested Exceeded the regulatory limit for residual DNA set by the US Food & Drug Administration (FDA) and the World Health Authorization (WHO) by: Pfizer: 36-153-fold Moderna: 112-627-fold 3 Pfizer vials exceeded the regulatory limit for the SV40 promoter-enhancer-ori (p53) The PCR results for the most recent XBB.1.5 Moderna and Pfizer vaccines suggest that DNA residues have not been reduced from previous vaccine versions. Pfizer, Total DNA ranged 371-1,548 ng/dose Moderna, 1,130-6,280 ng/dose Specific DNA of multiple plasmid DNA targets Pfizer ranged 0.22-7.28 ng/dose Moderna 0.01-0.78 ng/dose for Moderna. The SV40 promoter-enhancer-ori(0.25-23.72 ng/dose) was only detected in Pfizer vials. Sequencing of one vial Mean DNA length, 214 bp Maximum length, 3.5 kb Presence of 1.23 × 108 to 1.60 × 1011 plasmid DNA fragments per dose encapsulated in lipid nanoparticles. Our findings extend existing concerns about vaccine safety and call into question the relevance of guidelines conceived before the introduction of efficient transfection using LNPs. This work highlights the need for regulators and industry to adhere to the precautionary principle and provide sufficient and transparent evidence that products are safe and effective, and disclose the details of their composi…","meta_description":"Quantification of residual plasmid DNA and SV40 promoter-enhancer sequences in Pfizer/BioNTech and Moderna modRNA COVID-19 vaccines from Ontario, Canada h…","key_points":[],"chapters":[],"topics":[],"duration_seconds":1264,"processing_state":"not_requested","actions":[{"name":"request_transcript","method":"POST","url":"https://stenobird.com/v1/public/podcasts/dr-john-campbell-7851784/episodes/huge-dna-contamination/transcription-requests","description":"Idempotently request low-priority transcript generation for this episode."},{"name":"read_markdown","method":"GET","url":"https://stenobird.com/podcast/dr-john-campbell-7851784/huge-dna-contamination.md","description":"Read the agent-friendly Markdown representation of this episode resource."}]}}