{"podcast":{"title":"Concentrating on Chromatography","slug":"concentrating-on-chromatography-7157959","podcast_index_feed_id":7157959,"rss_url":"https://anchor.fm/s/fd632380/podcast/rss","website_url":"https://concentratingonchromatography.com","image_url":"https://d3t3ozftmdmh3i.cloudfront.net/staging/podcast_uploaded_nologo/42411328/42411328-1731191904094-fc5e6d90497be.jpg","author":"David Oliva","episode_count":62,"summary":"Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!","last_synced_at":null,"page_url":"https://stenobird.com/podcast/concentrating-on-chromatography-7157959"},"episode":{"title":"Episode 56: Fighting Antibiotic Resistance with LC-MS: Targeting Bacterial Cell Walls #chromatography #massspec","slug":"episode-56-fighting-antibiotic-resistance-with-lc-ms-targeting-bacterial-cell-walls-chromatography-massspec","published_at":"2026-04-13T10:00:00+00:00","page_url":"https://stenobird.com/podcast/concentrating-on-chromatography-7157959/episode-56-fighting-antibiotic-resistance-with-lc-ms-targeting-bacterial-cell-walls-chromatography-massspec","show_page_url":"https://stenobird.com/podcast/concentrating-on-chromatography-7157959","url":"https://podcasters.spotify.com/pod/show/chromatography/episodes/Episode-56-Fighting-Antibiotic-Resistance-with-LC-MS-Targeting-Bacterial-Cell-Walls-chromatography-massspec-e3hisnn","audio_url":"https://anchor.fm/s/fd632380/podcast/play/118108343/https%3A%2F%2Fd3ctxlq1ktw2nl.cloudfront.net%2Fstaging%2F2026-3-7%2F421607693-44100-2-231fe84a0d87b.m4a","summary":"In this episode of Concentrating on Chromatography, host Dave Oliva sits down with Chris Reid to explore how chemical biology and LC-MS are being used to tackle one of the most urgent global challenges: antimicrobial resistance.As antibiotic resistance continues to rise—and new drug development struggles to keep pace—researchers are looking beyond traditional approaches. Instead of targeting how bacteria build their cell walls, Chris’s work focuses on how they break them down, opening the door to entirely new antimicrobial strategies.--- 🔬 What You’ll Learn in This Episode:* Why antimicrobial resistance is accelerating globally* What peptidoglycan is and why it’s essential to bacterial survival* How targeting cell wall degradation differs from traditional antibiotics* The strategy behind designing small molecule probes and inhibitors* How LC-MS is used to analyze peptidoglycan fragments and enzyme activity* Key analytical challenges: complex mixtures, low-abundance species, and structural similarity* The importance of sample preparation and reproducibility in LC-MS workflows* How this research could lead to new antibiotic targets--- ⚗️ LC-MS in FocusThis episode dives deep into how LC-MS enables:* Detection of peptidoglycan fragment profiles* Analysis of cross-linking patterns via retention time and mass* Identification of structural changes using tandem mass spectrometryChris also shares practical insights on:* Sample prep challenges with insoluble biomaterials* Common pitfalls in LC-MS workflows* How to ensure consistent, reproducible data--- 🚀 Why This MattersWith bacteria rapidly evolving resistance to existing drugs, the need for new antimicrobial strategies has never been greater. This research highlights how combining chemistry, biology, and analytical science c…","meta_description":"In this episode of Concentrating on Chromatography, host Dave Oliva sits down with Chris Reid to explore how chemical biology and LC-MS are being used to…","key_points":[],"chapters":[],"topics":[],"duration_seconds":1305,"processing_state":"not_requested","actions":[{"name":"request_transcript","method":"POST","url":"https://stenobird.com/v1/public/podcasts/concentrating-on-chromatography-7157959/episodes/episode-56-fighting-antibiotic-resistance-with-lc-ms-targeting-bacterial-cell-walls-chromatography-massspec/transcription-requests","description":"Idempotently request low-priority transcript generation for this episode."},{"name":"read_markdown","method":"GET","url":"https://stenobird.com/podcast/concentrating-on-chromatography-7157959/episode-56-fighting-antibiotic-resistance-with-lc-ms-targeting-bacterial-cell-walls-chromatography-massspec.md","description":"Read the agent-friendly Markdown representation of this episode resource."}]}}