{"podcast":{"title":"Concentrating on Chromatography","slug":"concentrating-on-chromatography-7157959","podcast_index_feed_id":7157959,"rss_url":"https://anchor.fm/s/fd632380/podcast/rss","website_url":"https://concentratingonchromatography.com","image_url":"https://d3t3ozftmdmh3i.cloudfront.net/staging/podcast_uploaded_nologo/42411328/42411328-1731191904094-fc5e6d90497be.jpg","author":"David Oliva","episode_count":62,"summary":"Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!","last_synced_at":null,"page_url":"https://stenobird.com/podcast/concentrating-on-chromatography-7157959"},"episode":{"title":"Episode 46: How LA-ICP-MS Imaging Reveals Disease in Tissue | Metrology, Multiplexing & Analytical Chemistry","slug":"episode-46-how-la-icp-ms-imaging-reveals-disease-in-tissue-metrology-multiplexing-analytical-chemistry","published_at":"2026-02-09T12:00:00+00:00","page_url":"https://stenobird.com/podcast/concentrating-on-chromatography-7157959/episode-46-how-la-icp-ms-imaging-reveals-disease-in-tissue-metrology-multiplexing-analytical-chemistry","show_page_url":"https://stenobird.com/podcast/concentrating-on-chromatography-7157959","url":"https://podcasters.spotify.com/pod/show/chromatography/episodes/Episode-46-How-LA-ICP-MS-Imaging-Reveals-Disease-in-Tissue--Metrology--Multiplexing--Analytical-Chemistry-e3ehgnf","audio_url":"https://anchor.fm/s/fd632380/podcast/play/114917551/https%3A%2F%2Fd3ctxlq1ktw2nl.cloudfront.net%2Fstaging%2F2026-1-2%2F417318009-44100-2-347195fe274cc.m4a","summary":"How do you see proteins, metals, and disease processes inside real tissue — and still trust the numbers?In this episode of Concentrating on Chromatography, David sits down with Monique Mello, analytical chemist, educator, and LA-ICP-MS imaging specialist, to explore how laser ablation ICP-MS (LA-ICP-MS) and immuno-mass spectrometry imaging (iMSI) are transforming pathology, environmental science, and translational research.Monique shares her journey from public-health and pathology labs in Brazil to environmental and biomedical research in Australia — and explains why metrology, traceability, and defensible measurements are the foundation of meaningful science.We dive into her work developing multiplexed elemental imaging methods that allow researchers to quantify multiple proteins at once in tissue — revealing interactions that traditional single-marker methods miss. Her studies show how LA-ICP-MS can map dystrophin-glycoprotein complex proteins in muscular dystrophy and track elemental distributions like zinc in Alzheimer’s disease tissue.We also discuss something many labs overlook: sample preparation and immunolabelling can change the chemistry you’re trying to measure. Monique’s research demonstrates how staining steps can redistribute endogenous metals and why rigorous validation is critical for trustworthy data.If you care about chromatography, mass spectrometry, or analytical chemistry that genuinely impacts patients and communities, this episode is for you.In this conversation, we cover:• What LA-ICP-MS imaging is and how it works• Multiplexed antibody tagging with lanthanides for quantitative tissue imaging• Why metrology and uncertainty matter more than “pretty data”• Common analytical failures (and why sample prep causes most of them)• Elemental mapping in…","meta_description":"How do you see proteins, metals, and disease processes inside real tissue — and still trust the numbers?In this episode of Concentrating on Chromatography…","key_points":[],"chapters":[],"topics":[],"duration_seconds":2135,"processing_state":"not_requested","actions":[{"name":"request_transcript","method":"POST","url":"https://stenobird.com/v1/public/podcasts/concentrating-on-chromatography-7157959/episodes/episode-46-how-la-icp-ms-imaging-reveals-disease-in-tissue-metrology-multiplexing-analytical-chemistry/transcription-requests","description":"Idempotently request low-priority transcript generation for this episode."},{"name":"read_markdown","method":"GET","url":"https://stenobird.com/podcast/concentrating-on-chromatography-7157959/episode-46-how-la-icp-ms-imaging-reveals-disease-in-tissue-metrology-multiplexing-analytical-chemistry.md","description":"Read the agent-friendly Markdown representation of this episode resource."}]}}