# Phlebotomy Exam Prep 43, Centrifugation Principles and Time

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Text version: https://stenobird.com/podcast/nursing-healthcare-exam-prep-7652377/phlebotomy-exam-prep-43-centrifugation-principles-and-time.md
Podcast: [Nursing & Healthcare Exam Prep](https://stenobird.com/podcast/nursing-healthcare-exam-prep-7652377)
Published: 2026-06-09T22:25:11+00:00
Episode link: https://open-exam-prep.com/
Audio file: https://episodes.captivate.fm/episode/9bb357ca-5814-4ef5-ae84-ac3c31e10b17.mp3
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Duration seconds: 200

## Resource

This podcast is made by Ran Chen, who holds an EA license, Insurance and Securities licenses (Series 6, 63, 65), and the CFP® designation. He is passionate about opening access to high-quality exam preparation resources and helping learners prepare more effectively for professional certification exams. In this episode you will learn: - The standard centrifugation setting for serum and plasma is 10-15 minutes at a Relative Centrifugal Force (RCF) of 1000 to 1300g. - Why Serum Separator Tubes (SSTs) must be allowed to clot for a full 30 minutes at room temperature before being placed in the centrifuge. - The critical importance of precisely counterbalancing the centrifuge to ensure sample integrity, prevent mechanical damage, and avoid creating biohazardous aerosols. - The absolute rule against re-spinning gel separator tubes and the associated risk of falsely elevated potassium levels. - How using the centrifuge's hard brake can disrupt cellular separation and lead to hemolysis, and why letting it coast to a stop is the correct procedure. For more free exam prep tools, practice questions, and AI-powered explanations, visit https://open-exam-prep.com/ or YouTube Channel: https://www.youtube.com/@Open-exam-prep

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## Transcript

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