# Huge DNA contamination

Page: https://stenobird.com/podcast/dr-john-campbell-7851784/huge-dna-contamination
Text version: https://stenobird.com/podcast/dr-john-campbell-7851784/huge-dna-contamination.md
Podcast: [Dr. John Campbell](https://stenobird.com/podcast/dr-john-campbell-7851784)
Published: 2026-07-13T01:30:00+00:00
Episode link: https://traffic.megaphone.fm/VG6622757007.mp3
Audio file: https://traffic.megaphone.fm/VG6622757007.mp3
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JSON: https://stenobird.com/v1/public/podcasts/dr-john-campbell-7851784/episodes/huge-dna-contamination
Duration seconds: 1264

## Resource

Quantification of residual plasmid DNA and SV40 promoter-enhancer sequences in Pfizer/BioNTech and Moderna modRNA COVID-19 vaccines from Ontario, Canada https://pubmed.ncbi.nlm.nih.gov/40913499/ 32 vials representing 16 unique vaccine lots. These data demonstrate the presence of billions to hundreds of billions of DNA molecules per dose in the modRNA COVID-19 products tested. All products tested exceeded the guidelines for residual DNA set by the FDA and WHO of 10ng/dose by 36–627-fold. Total DNA in all vials tested Exceeded the regulatory limit for residual DNA set by the US Food & Drug Administration (FDA) and the World Health Authorization (WHO) by: Pfizer: 36-153-fold Moderna: 112-627-fold 3 Pfizer vials exceeded the regulatory limit for the SV40 promoter-enhancer-ori (p53) The PCR results for the most recent XBB.1.5 Moderna and Pfizer vaccines suggest that DNA residues have not been reduced from previous vaccine versions. Pfizer, Total DNA ranged 371-1,548 ng/dose Moderna, 1,130-6,280 ng/dose Specific DNA of multiple plasmid DNA targets Pfizer ranged 0.22-7.28 ng/dose Moderna 0.01-0.78 ng/dose for Moderna. The SV40 promoter-enhancer-ori(0.25-23.72 ng/dose) was only detected in Pfizer vials. Sequencing of one vial Mean DNA length, 214 bp Maximum length, 3.5 kb Presence of 1.23 × 108 to 1.60 × 1011 plasmid DNA fragments per dose encapsulated in lipid nanoparticles. Our findings extend existing concerns about vaccine safety and call into question the relevance of guidelines conceived before the introduction of efficient transfection using LNPs. This work highlights the need for regulators and industry to adhere to the precautionary principle and provide sufficient and transparent evidence that products are safe and effective, and disclose the details of their composi…

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